iciA, an Escherichia coli gene encoding a specific inhibitor of chromosomal initiation of replication in vitro.
Identifieur interne : 004859 ( Main/Exploration ); précédent : 004858; suivant : 004860iciA, an Escherichia coli gene encoding a specific inhibitor of chromosomal initiation of replication in vitro.
Auteurs : B. Thöny [États-Unis] ; D S Hwang ; L. Fradkin ; A. KornbergSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1991.
Descripteurs français
- KwdFr :
- Cartographie de restriction, Chromosomes de bactérie, Clonage moléculaire, Données de séquences moléculaires, Escherichia coli (génétique), Expression des gènes, Gènes bactériens, Hybridation d'acides nucléiques, Protéines Escherichia coli, Protéines bactériennes (génétique), Protéines de liaison à l'ADN (génétique), Réaction de polymérisation en chaîne, Réplication de l'ADN, Similitude de séquences d'acides nucléiques, Séquence d'acides aminés, Séquence nucléotidique.
- MESH :
- génétique : Escherichia coli, Protéines bactériennes, Protéines de liaison à l'ADN.
- Cartographie de restriction, Chromosomes de bactérie, Clonage moléculaire, Données de séquences moléculaires, Expression des gènes, Gènes bactériens, Hybridation d'acides nucléiques, Protéines Escherichia coli, Réaction de polymérisation en chaîne, Réplication de l'ADN, Similitude de séquences d'acides nucléiques, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Bacterial Proteins (genetics), Base Sequence, Chromosomes, Bacterial, Cloning, Molecular, DNA Replication, DNA-Binding Proteins (genetics), Escherichia coli (genetics), Escherichia coli Proteins, Gene Expression, Genes, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, Polymerase Chain Reaction, Restriction Mapping, Sequence Homology, Nucleic Acid.
- MESH :
- chemical , genetics : Bacterial Proteins, DNA-Binding Proteins.
- genetics : Escherichia coli.
- Amino Acid Sequence, Base Sequence, Chromosomes, Bacterial, Cloning, Molecular, DNA Replication, Escherichia coli Proteins, Gene Expression, Genes, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, Polymerase Chain Reaction, Restriction Mapping, Sequence Homology, Nucleic Acid.
Abstract
The gene encoding the protein that binds the three 13-mers in the origin (oriC) of Escherichia coli to block initiation of replication in vitro has been cloned, sequenced, and overexpressed. The gene possesses an open reading frame for 297 amino acids (mass of 33,471 Da). The protein has a motif for DNA-binding (helix-turn-helix) and has homology to a diverse set of prokaryotic regulatory proteins, known as the LysR family. The protein, previously referred to as the 33-kDa protein, has been named IciA (for inhibitor of chromosome initiation). The iciA gene is at 62.8 min on the chromosomal map. Cells with enhanced levels of the protein grow at a normal rate but generally exhibit a pronounced lag upon transfer to a fresh medium.
DOI: 10.1073/pnas.88.10.4066
PubMed: 2034653
Affiliations:
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Le document en format XML
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Fradkin</name></author><author><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1991">1991</date><idno type="RBID">pubmed:2034653</idno><idno type="pmid">2034653</idno><idno type="doi">10.1073/pnas.88.10.4066</idno><idno type="wicri:Area/PubMed/Corpus">002997</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002997</idno><idno type="wicri:Area/PubMed/Curation">002997</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002997</idno><idno type="wicri:Area/PubMed/Checkpoint">002819</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002819</idno><idno type="wicri:Area/Ncbi/Merge">000748</idno><idno type="wicri:Area/Ncbi/Curation">000748</idno><idno type="wicri:Area/Ncbi/Checkpoint">000748</idno><idno type="wicri:doubleKey">0027-8424:1991:Thony B:icia:an:escherichia</idno><idno type="wicri:Area/Main/Merge">004933</idno><idno type="wicri:Area/Main/Curation">004859</idno><idno type="wicri:Area/Main/Exploration">004859</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">iciA, an Escherichia coli gene encoding a specific inhibitor of chromosomal initiation of replication in vitro.</title><author><name sortKey="Thony, B" sort="Thony, B" uniqKey="Thony B" first="B" last="Thöny">B. Thöny</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, Stanford University School of Medicine, CA 94305-5307.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Biochemistry, Stanford University School of Medicine</wicri:cityArea></affiliation></author><author><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name></author><author><name sortKey="Fradkin, L" sort="Fradkin, L" uniqKey="Fradkin L" first="L" last="Fradkin">L. Fradkin</name></author><author><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Bacterial Proteins (genetics)</term><term>Base Sequence</term><term>Chromosomes, Bacterial</term><term>Cloning, Molecular</term><term>DNA Replication</term><term>DNA-Binding Proteins (genetics)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli Proteins</term><term>Gene Expression</term><term>Genes, Bacterial</term><term>Molecular Sequence Data</term><term>Nucleic Acid Hybridization</term><term>Polymerase Chain Reaction</term><term>Restriction Mapping</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cartographie de restriction</term><term>Chromosomes de bactérie</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Escherichia coli (génétique)</term><term>Expression des gènes</term><term>Gènes bactériens</term><term>Hybridation d'acides nucléiques</term><term>Protéines Escherichia coli</term><term>Protéines bactériennes (génétique)</term><term>Protéines de liaison à l'ADN (génétique)</term><term>Réaction de polymérisation en chaîne</term><term>Réplication de l'ADN</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>DNA-Binding Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Protéines bactériennes</term><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Chromosomes, Bacterial</term><term>Cloning, Molecular</term><term>DNA Replication</term><term>Escherichia coli Proteins</term><term>Gene Expression</term><term>Genes, Bacterial</term><term>Molecular Sequence Data</term><term>Nucleic Acid Hybridization</term><term>Polymerase Chain Reaction</term><term>Restriction Mapping</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term><term>Chromosomes de bactérie</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Gènes bactériens</term><term>Hybridation d'acides nucléiques</term><term>Protéines Escherichia coli</term><term>Réaction de polymérisation en chaîne</term><term>Réplication de l'ADN</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The gene encoding the protein that binds the three 13-mers in the origin (oriC) of Escherichia coli to block initiation of replication in vitro has been cloned, sequenced, and overexpressed. The gene possesses an open reading frame for 297 amino acids (mass of 33,471 Da). The protein has a motif for DNA-binding (helix-turn-helix) and has homology to a diverse set of prokaryotic regulatory proteins, known as the LysR family. The protein, previously referred to as the 33-kDa protein, has been named IciA (for inhibitor of chromosome initiation). The iciA gene is at 62.8 min on the chromosomal map. Cells with enhanced levels of the protein grow at a normal rate but generally exhibit a pronounced lag upon transfer to a fresh medium.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Fradkin, L" sort="Fradkin, L" uniqKey="Fradkin L" first="L" last="Fradkin">L. Fradkin</name><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Thony, B" sort="Thony, B" uniqKey="Thony B" first="B" last="Thöny">B. Thöny</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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